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ExoClean and ExoTracker
K-4300a, K-4300b, K-4300c, K-4300d (20 tests)
Description: Simple, quick protocols for labeling (ExoTracker) and isolating (ExoClean) extracellular vesicles (EVs) from biological samples*
Echelon’s ExoClean – EV Purification Kit and ExoTracker kits为标记和纯化外显体和细胞外囊泡（EV）提供了一个简单可靠的系统。（1）The ExoClean – EV Purification Kit (货号：K-4300a )通过将沉淀试剂与微离心柱配对，取代了与基于超离心的纯化相关的时间和设备成本，此外可以去除与其他沉淀技术相关的有害污染物。
（2）The ExoTracker kits (货号：K-4300b -d) 使该系统的实用性更进一步，可以用膜或RNA特异性标签标记EV。
产品列表
Catalog # | Description | Quantity |
ExoClean (K-4300a)
K-4301 | EV Precipitation Reagent | 30 mL |
K-SPIN | Microcentrifuge Columns | 20 columns |
ExoTracker - Membrane (K-4300b)
K-4300a | ExoClean | 1 kit |
K-4302 | EV Membrane Label | 150 µg |
ExoTracker - RNA (K-4300c)
K-4300a | ExoClean | 1 kit |
K-4303 | EV RNA Label | 20 µL |
ExoTracker - Total (K-4300d)
K-4300a | ExoClean | 1 kit |
K-4302 | EV Membrane Label | 150 µg |
K-4303 | EV RNA Label | 20 µL |
操作流程：
1：Collect EV sample
2：Centrifuge at 2000g for 10 min to remove any debris or cells.
3：Transfer supernatant and precipitate cleared sample with-K4301 for 30 minutes at room temperature with mixing
4：Centrifuge precipitated sample at 1500g for 30 minutes at room temperature.
5：Discard the supernatant fraction and resuspend the precipitated pellet in 1x PBS.
6：Apply the resuspended pellet to the equilibrated K-SPINcolumn and incubate for 5 minutes at room temperature.
7：Centrifuge the column at 500g for 30 seconds at room temperature.
8：Collect and examine the eluted, purified EVs.
注：Exosomes and extracellular vesicles (EVs)-胞外体和胞外小泡
发表文献：
1. Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of Extracellular Vesicles: General Methodologies and Latest Trends. Biomed Res Int. 2018;2018:8545347.
2. Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in Exosome Isolation Techniques. Theranostics. 2017;7(3):789-804.
3. Deregibus MC, Figliolini F, D’Antico S, Manzini PM, Pasquino C, De Lena M, et al. Charge-based precipitation of extracellular vesicles. Int J Mol Med. 2016;38(5):1359-66.
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